Molecular Formula | C23H46N6O13 |
Molar Mass | 614.64 |
Density | 1.3548 (rough estimate) |
Melting Point | >194°C (dec.) |
Boling Point | 658.4°C (rough estimate) |
Solubility | Aqueous Acid (Slightly), Water (Slightly) |
Appearance | liquid |
Color | clear |
Merck | 13,6482 |
pKa | 12.93±0.70(Predicted) |
Storage Condition | 2-8°C |
Stability | Hygroscopic |
Refractive Index | 1.6000 (estimate) |
In vitro study | The inhibition of RNase P RNA cleavage by Framycetin (Neomycin B; Fradiomycin B) is sensitive to pH and an increase in pH suppresses the inhibition in other systems. Framycetin targets the bacterial and human ribosome and affect translation. 5″-azido neomycin B and Framycetin selectively inhibit production of the mature miRNA, boosts a downstream protein, and inhibits invasion in HCC cell line. Framycetin binds to a structural rather than a sequence motif of the RNA. Its primary cognate target is the decoding site of the 16S rRNA, but it also binds to the Rev-responsive element in HIV-1, group I introns, and the hammerhead ribozyme, and thus inhibits their biological function. Framycetin induces misreading of the genetic code during translation and inhibits several ribozymes. The ribosomal target site is the 16 S rRNA 1400 to 1500 region. |
Hazard Symbols | Xn - Harmful |
Risk Codes | R42/43 - May cause sensitization by inhalation and skin contact. R63 - Possible risk of harm to the unborn child R36/37/38 - Irritating to eyes, respiratory system and skin. |
Safety Description | S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36/37/39 - Wear suitable protective clothing, gloves and eye/face protection. S45 - In case of accident or if you feel unwell, seek medical advice immediately (show the label whenever possible.) S36/37 - Wear suitable protective clothing and gloves. S23 - Do not breathe vapour. |
WGK Germany | 2 |
FLUKA BRAND F CODES | 8 |
1mg | 5mg | 10mg | |
---|---|---|---|
1 mM | 1.627 ml | 8.135 ml | 16.269 ml |
5 mM | 0.325 ml | 1.627 ml | 3.254 ml |
10 mM | 0.163 ml | 0.813 ml | 1.627 ml |
5 mM | 0.033 ml | 0.163 ml | 0.325 ml |
biological activity | framinectin (Neomycin B) is an aminoglycoside antibiotic, it is a potent inhibitor of RNase P cleavage activity with a Ki of 35 μm. Framyce tin competes for specific divalent metal ion binding sites in RNase P RNA. Framinectin inhibits hammerhead ribozyme with a Ki value of 13.5 μm. Framyce tin is a 5 "-azido neomycin B precursor that binds to the Drosha site in miR-525 and is useful for hepatic encephalopathy and enteropathogenic E. Coli infection. |
Target | Ki: 35 μm (RNase P cleavage activity) and 13.5 μm (hammerhead ribozyme) |